Molecular Formula | C27H46ClNO2 |
Molar Mass | 452.11 |
Melting Point | 281-284°C (dec.) |
Solubility | Methanol (Slightly) |
Appearance | Powder |
Color | White to Off-White |
BRN | 3920740 |
Storage Condition | 2-8°C |
In vitro study | Tomatidine decreases inducible NO synthase and COX-2 expression through suppression of I-κBα phosphorylation, NF-κB nuclear translocation and JNK activation, which in turn inhibits c-jun phosphorylation and Oct-2 expression. Tomatidine, solasodine and diosgenin (40 μM) show 66%, 22% and 41% inhibition of nitrite production, respectively. The iNOS protein is barely detectable in unstimulated cells but markedly increases after LPS treatment, and Tomatidine causes dose-dependent inhibition of LPS-induced iNOS expression. p65 is the major component of NF-κB in LPS-stimulated macrophages, the effect of Tomatidine on p65 DNA-binding activity is determined. In the presence of Tomatidine at 10-40 μM, the binding activity of NF-κB is suppressed in a dose-dependent manner. Tomatidine inhibits the phosphorylation of I-κB, blocks the I-κB production, and furthermore suppresses p65 NF-κB translocation to the nucleus and modulated binding activity. |
Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. |
WGK Germany | 3 |
biological activity | Tomatidine hydrochloride exerts anti-inflammatory effects by blocking NF-κB and JNK signaling. Tomatidine hydrochloride activates autophagy in mammalian cells or Caenorhabditis elegans. |
Use | for content determination/identification/pharmacological experiments. |